PY2160: pY Pichia Secretory SUMOstar Expression Kit

SKU PY-2160-1000 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

Lifesensors PY2160 is a pY Pichia Secretory SUMOstar Expression Kit enabling high-yield, soluble protein expression with easy purification and efficient tag removal for research use

$1,947.00

In stock

The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His6 tag precede SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of specific endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

pY-secSUMOstar vector (Cat.# 2161) — 20µg

SUMOstar Protease (Cat.# 4110) — 500 Units

SUMOstar Protease control protein (Cat.# 5010) — 100µg

Anti-SUMOstar antibody (Cat.# AB7002) — 50µg

Info

Fusion tag	His6-SUMOstar
Quantity	N/A
Promoter	AOX1
Selection marker	Ampicillin (E. coli), Zeocin (P. pastoris)
Host	E. coli, P. pastoris
Storage	Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

Applications

Enhancing functional protein expression in P. Pastoris.

Benefits

SUMOstar fusion often enhances expression.

SUMOstar fusion often enhances protein solubility.

No known case of SUMOstar Protease cleaving within the fused protein-of-interest.

Cleavage yields native protein with a desired N-terminus.

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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PY2160: pY Pichia Secretory SUMOstar Expression Kit

Components:

Info

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