PY2101: pY-SUMOstar Saccharomyces Intracellular Vector

LifeSensors PY2101 is a pY-SUMOstar Saccharomyces intracellular vector for efficient intracellular expression of SUMOstar fusion proteins in yeast, enhancing protein yield and solubility.

$475.00

SKU PY-2101-0020 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

In stock

Product Advantages

Applications

Enhanced intracellular expression of recombinant proteins as SUMOstar fusion proteins
Analysis of SUMOstar fusion protein expression

Removal of SUMOstar fusion tag for purification of native protein of interest

Benefits

SUMOstar enhances expression compared to traditional gene fusion systems.
Cleavage generates native protein, with desired N-terminus (except proline).

The pY-SUMOstar vector allows for the intracellular expression of SUMOstar fusion proteins in Saccharomyces cerevisiae. SUMOstar fusion constructs are not processed in vivo, allowing for the same enhancement of protein expression, solubility, and stability afforded by SUMOpro.Protein expression is driven by the S. cerevisiae copper metallothionein (CUP1) promoter, which is both tightly regulated and highly inducible. The plasmid is maintained by the 2-micron element and selected for by the restoration of L-tryptophan biosynthesis in Trp auxotrophs. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

pY-SUMOstar vector (Cat.# 2101) — 20 µg

Info

Fusion tag	His6-SUMOstar
Quantity	n/a
Promoter	CUP1
Selection marker	Ampicillin (E. coli), Trp1 (yeast)
Host	E. coli, yeast
Storage	Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.