PY2101: pY-SUMOstar Saccharomyces Intracellular Vector

SKU PY-2101-0005 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

LifeSensors’ PY2101 is a pY-SUMOstar Saccharomyces intracellular vector for efficient intracellular expression of SUMOstar fusion proteins in yeast, enhancing protein yield and solubility

$475.00

In stock

The pY-SUMOstar vector allows for the intracellular expression of SUMOstar fusion proteins in Saccharomyces cerevisiae. SUMOstar fusion constructs are not processed in vivo, allowing for the same enhancement of protein expression, solubility, and stability afforded by SUMOpro. Protein expression is driven by the S. cerevisiae copper metallothionein (CUP1) promoter, which is both tightly regulated and highly inducible. The plasmid is maintained by the 2-micron element and selected for by the restoration of L-tryptophan biosynthesis in Trp auxotrophs. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

Fusion tag	His6-SUMOstar
Quantity	5µg
Promoter	CUP1
Selection marker	Ampicillin (E. coli), Trp1 (yeast)
Host	E. coli, yeast
Storage	Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

Enhanced intracellular expression of recombinant proteins as SUMOstar fusion proteins
Analysis of SUMOstar fusion protein expression
Removal of SUMOstar fusion tag for purification of native protein of interest

Benefits

SUMOstar enhances expression compared to traditional gene fusion systems
Cleavage generates native protein, with desired N-terminus (except proline)

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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PY2101: pY-SUMOstar Saccharomyces Intracellular Vector

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