PY2100: pY-SUMOstar Saccharomyces Intracellular Expression Kit

SKU PY-2100-1000 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

LifeSensors’ PY2100 is a pY-SUMOstar Saccharomyces intracellular expression kit for efficient SUMOstar fusion protein production in yeast cells

$1,947.00

In stock

In eukaryotes, endogenous SUMO proteases rapidly cleave SUMO fusions in vivo, precluding tag-enhanced expression and purification capacities. To circumvent this problem, LifeSensors has engineered the SUMOstar fusion system. SUMOstar offers the same solubility and expression enhancing benefits as the original wild-type SUMO tag; however, the tag is not cleaved by endogenous eukaryotic SUMO proteases. In addition, LifeSensors has created a cognate SUMOstar protease for specific recognition and cleavage of the SUMOstar tag in vitro, liberating the protein-of-interest with a desired N-terminal amino acid. The pY-Saccharomyces Intracellular SUMOstar Kit allows for expression of SUMOstar fusion proteins intracellularly. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Components:

pY-SUMOstar vector (Cat.# 2101) — 20µg
SUMOstar Protease 1 (Cat.# 4110) — 500 Units
SUMOstar Protease control protein (Cat. #5010) — 100µg
Anti-SUMO/SUMOstar antibody (Cat.# AB7002) — 50µg

Info

Fusion tag	His6-SUMOstar
Quantity	N/A
Promoter	CUP1
Selection marker	Ampicillin, Trp1
Host	E. coli, yeast
Storage	Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.

Enhanced intracellular expression of recombinant proteins as SUMOstar fusion proteins
Analysis of SUMOstar fusion protein expression

Removal of SUMOstar fusion tag for purification of native protein of interest

Benefits

SUMOstar enhances expression compared to traditional gene fusion systems

Cleavage generates native protein, with desired N-terminus (except proline)

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

Phone (Office)

610.644.8845

Available Monday – Friday, 9:00 AM – 5:00 PM EST

Send Email

info@lifesensors.com

We typically respond within 24 hours

Visit Anytime

271 Great Valley Parkway Malvern, PA 19355

Send us a Message

Have a question about our solutions? We’d love to hear from you.

First Name *

Last Name *

Email Address *

Phone Number

Company/Organization

Subject*

Message *

I would like to receive updates about new products and research developments

* Required fields

Custom Solutions

Learning Center

PY2100: pY-SUMOstar Saccharomyces Intracellular Expression Kit

Components:

Info

Benefits

Follow Us

Send us a Message

More from This Category

PROTAC is a registered trademark of Arvinas Operations, Inc., and is used under license.

Menu

Custom Solutions

Learning Center