SP4110: SUMOstar Protease

$458.00 – $6,103.00

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SKU: SP-4110 Categories: , ,

Specifications

Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems (Cat.#s: 1100A, 1106, 1100K, 1101, 2100, 2101, 2160, 2161, 3100, 3101, 7110, 7111, 7120, 7121). Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

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Info

Species Yeast
Source E. coli
Tag His6 
Molecular Weight 26.4 kDa
Quantity 250, 500, 1000, 5000 units
Concentration Variable
Formulation 50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Units

250 UNITS, 500 UNITS, 1000 UNITS, 5000 UNITS

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  • SUMOstar Protease 1 contains a polyhistidine tag at the N-terminus, allowing easy removal from the cleavage reaction by affinity chromatography. The protease cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5-9.5], and ionic strength.
  • Specifically engineered to recognize and cleave the SUMOstar fusion tag

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