Questions about a product, technology or experimental design? Give us a call 610-644-8845
Questions about a product, technology or experimental design? Give us a call 610-644-8845.

PA770C: PROTAC® In Vitro Ubiquitination Assay Kit (Colorimetric)

LifeSensors’ PA770 is a PROTAC® In Vitro Ubiquitination Assay Kit for high-throughput screening of PROTAC activity by monitoring target protein ubiquitination with E3 ligases VHL, Cereblon, and HDM2.

$1,045.00

Proteolysis Targeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that both simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase. This subsequently leads to ubiquitination and degradation of the target protein. The In vitro ubiquitination kit has been developed to establish a high throughput approach that can accurately predict PROTAC efficiency by monitoring the protein’s intrinsic ability to get ubiquitinated. We offer this kit for three E3 ubiquitin ligases: Cereblon, VHL, and HDM2 to monitor PROTAC mediated ubiquitination for target of choice. New assay plate developed for colorimetric detection allows for quantitative measurement using absorbance-based plate readers. Colorimetric readouts (650nm, 480nm) are more consistent day-to-day than luminescent-based detection methods, fast, and easy to use.
For evaluating molecular glues, we recommend using our MG780: Molecular Glue In Vitro Ubiquitination Assay Kit. This kit is specially designed to measure the performance of molecular glues In vitro.
PROTAC In Vitro Ubiquitination Assay Kit Components
In Vitro ubiquitination Assay Plate 1 plate
Assay Buffer (10X) 1.2mL
E3 Mix (20X) 1 X 250µL
E1-E2-Ubiquitination Mix (20X) 1 X 250µL
Secondary Antibody (Anti-Mouse or Anti-Rabbit HRP conjugate) 60µL (100X)
Colorimetric Reagent (CR)Stopping Reagent (SR) CR- 5mLSR- 5mL
Blocking Concentrate (5X) 5mL
PROTAC in DMSO 10µL
Target Protein positive control BRD3 (20X) 20µL
Anti-BRD3 antibody (100X) 5µL
Anti-mouse HRP Conjugate (100X) 5µL
This product has been cited 1 time. Need guidance or technical assistance with LifeSensors’ PROTAC Assay Plate? We’re happy to help! Contact our technical support team at customerservice@lifesensors.com. Learn more about PROTACs here. Screening Ubiquitin application here.
  • Testing PROTAC and molecular glue (MGs) activity
  • Compare between multiple PROTAC variants and establish predictive DC50 from UbMax
  • Choose all three ligases for comparing PROTACs / MGs activity to demonstrate selectivity
  • Test PROTAC substrate specificity and Isoform selectivity

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

Available Monday – Friday, 9:00 AM – 5:00 PM EST
We typically respond within 24 hours

Visit Anytime

271 Great Valley Parkway Malvern, PA 19355

Send us a Message

Have a question about our solutions? We’d love to hear from you.
* Required fields

More from This Category

LifeSensors' PA950 PROTAC Assay Plate Kit: Optimized for drug discovery, this assay kit enables efficient screening of PROTACs, enhancing proteolysis-targeted....
LifeSensors' PC1008: LS E3 1017 is a thalidomide derivative used as a precursor for PROTACs targeting cereblon E3 ubiquitin ligase....
LifeSensors' PC1005 is a specialized assay kit designed for ubiquitin pathway enzyme research, enabling precise analysis of ubiquitin ligases and....

PROTAC is a registered trademark of Arvinas Operations, Inc., and is used under license.

Menu

Learning Center