K6 Tri-Ubiquitin (Ub3) Mutant

LifeSensor’s K6 Tri-Ubiquitin (Ub3) Mutant is a high-purity, enzymatically linked tri-ubiquitin chain for studying mitophagy, DNA repair, and immune response pathways.

SKU SI-0623 Categories All, Ubiquitin and Neuro-degeneration, Ubiquitin Chains and Derivatives Tag Degradation

Product Advantages

Application

To investigate enzymes that cleave this specific peptide linkage between two ubiquitin molecules.
To better understand the mechanisms of ubiquitin-activating (E1) or ubiquitin-conjugating (E2) enzymes, ubiquitin-associated domains (UBA), or ubiquitin-interacting motifs (UIMs) among others.
To use as a label through biotinylation or fluorescence.

Mitophagy (the autophagic processing of damaged mitochondria) via Parkin activation is mainly promoted by K6 and K63 linked chains. K6-linked ubiquitination is also a main contributor to the DNA damage response and acts as a DNA-binding enhancer during the innate immune system response. These chain types are also involved in protein stabilization and other non-degradative processes. The serine in position 20 has been changed to a cysteine. These tri-ubiquitin chains are generated from the enzymatic linkage of wild-type ubiquitin through lysine 6. Phospho-Ubiquitin (pS65) is enzymatically generated with PINK1 kinase from Red Flour Beetle (Tribolium castaneum).

Product Information

Purity	≥ 80% SDS-PAGE and Mass Spectrometry
Molecular Weight	25704 Da
Physical State	Liquid
Quantity	25 µg or 50 µg
Solubility	>30 mg/ml
Formulation	PBS pH 7.4, 5mM TCEP
Storage	-80°C, avoid freeze/thaw cycles

Document 1

Document 1

Application

To investigate enzymes that cleave this specific peptide linkage between two ubiquitin molecules.
To better understand the mechanisms of ubiquitin-activating (E1) or ubiquitin-conjugating (E2) enzymes, ubiquitin-associated domains (UBA), or ubiquitin-interacting motifs (UIMs) among others.
To use as a label through biotinylation or fluorescence.