UM302: TUBE 2 (Biotin)

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state. Biotin-TUBEs allow for the detection of polyubiquitin and polyubiquitylated proteins by ligand blotting (“far Western”) without heating the membrane. This reagent is a superior alternative to traditional polyubiquitin immunodetection techniques, such as anti-ubiquitin IgGs. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

Tag	Biotin
Molecular Weight	38.5 kDa + Biotin
Physical State	Liquid
Quantity	200µg
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles

*Biotinylation is restricted to the His₆-SUMO tag to avoid compromising the affinity of the TUBE moiety for polyubiquitin.