AB120: Anti-Ubiquitin Antibody (MAb; FK2)

Specifications

The FK2 monoclonal ubiquitin antibody is a frequently used and widely-cited ubiquitin antibody that detects both mono- and polyubiquitin, but not free ubiquitin. Raised against a crude preparation of polyubiquitinylated lysozyme, it has been shown to recognize K11-, K29-, K48- and K63-linked polyubiquitin and to perform in Western blotting, immunoprecipitation, and immunostaining applications. For comparative studies, LifeSensors has developed several monoclonal antibodies that recognize polyubiquitylated proteins and free ubiquitin. Like FK2, the VU-1 Ubiquitin Monoclonal Antibody has been shown to recognize mono-ubiquitin and all types of poly-ubiquitin chain linkages and is an excellent reagent to use for immunofluorescence in tissue sections and cell culture. If you are not performing immunoprecipitation experiments, the VU-1 antibody is a better choice.

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