Nanobodies and Single Chain Antibodies

Revolutionizing protein expression with advanced SUMO technology for superior therapeutic outcomes

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Mammalian cells no doubt became the factories of recombinant protein production, especially for antibodies. However, production of therapeutic proteins, such as cytokines, chemokines, and growth factors remains a problem, especially when the desired N-terminus is required for biological activity.

SUMO fusion system has emerged as an ideal way to increase production of proteins: SUMOpro for E.coli and SUMOstar for mammalian cells.

SUMO (Small Ubiquitin-like Modifier) is a member of the ubiquitin family, composed of a flexible N-terminal region followed by a ball-shaped ubiquitin-like fold.

Hydrophobic Core

Improves correct folding of the fused polypeptides

Hydrophilic Surface

Keeps the partner protein soluble

Precise Cleavage

SUMO protease recognizes SUMO structure to cleave at the junction

It is important to understand why the SUMO system has proven to be the best among all other fusion systems. The chaperoning properties of SUMO promote correct folding and protect the fusion partner from degradation. These factors contribute to dramatically enhance protein production and improve the quality of the fusion partner.

Key Advantages
Most cost-effective way to generate desired N-terminus of proteins
Nature-designed chaperoning properties captured for protein production
Efficient SUMO removal reduces cost of goods
Process easily adapted for downstream GMP production

Cloning Strategy

Expert guidance on optimal SUMO tag selection

Expression Systems

Selection of best strains and cell lines

Process Development

Non-GMP to GMP production pathway

Expression of Therapeutic Proteins in Mammalian Cells

The SUMO pathway is conserved in eukaryotic cells. E.coli lacks the SUMO pathway and SUMO proteases. Unlike SUMOpro that is used to express SUMO fusion in E.coli, native SUMO fusions are rapidly cleaved in eukaryotic cells.

To circumvent rapid cleavage in eukaryotic cells and to take advantage of chaperoning properties of SUMO, the SUMO tag is engineered to preserve enhanced protein production properties. SUMOstar fusions are not cleaved by endogenous eukaryotic SUMO proteases, and can be efficiently cleaved by SUMOstar protease in vitro.

LifeSensors offers both SUMOpro and SUMOstar for expression of chemokines, cytokines, and growth factors. Over 1000 proteins have been expressed as SUMOpro or SUMOstar fusions.

Fast Evaluation

Results delivered in 2-4 weeks

Therapeutic Protein Expression Presentation

Comprehensive guide to SUMO technology applications

To use, simply utilize the drop-down arrows in order to search for your target of interest or application category. LifeSensors SUMO technology has been utilized for the expression of many different targets and continues to be utilized worldwide.