PR1105: ISG15-CHOP2 Reporter System

The ISG15-CHOP2-Reporter DeISGylation Assay uses an ISG15-reporter fusion that is inactive until cleaved by an isopeptidase. Cleavage activates the reporter, which acts on its substrate, producing a signal proportional to isopeptidase activity.

$1,495.00

SKU PR-1105-0375 Categories CHOP2 Reporter Assays, DUBs

In stock

Product Advantages

Demonstration of novel deISGylating activity
High throughput screening (HTS) for antagonists or agonists of deISGylating activity

The ISG15-CHOP2-Reporter DeISGylation Assay consists of ISG15 fused to a reporter enzyme, as well as a separate reagent substrate for the reporter enzyme. When fused to ISG15, the reporter is rendered catalytically inactive. Following cleavage of the ISG15-reporter system by the isopeptidase, the free (and now active) reporter subsequently acts upon its substrate. Thus, in the coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply with these terms.

ISG15-CHOP2-Reporter System: 1 x 375 μL (4 μM)
PLpro (Control Isopeptidase): 2 x 10 μL (2 μM)
Reporter Substrate 2: 1 x 30 μL (25 μM)
Control Fluorophore 2: 1 x 20 μL (1 mM)