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UE903C: UbiQuant™ S Plate (Colorimetric)
LifeSensors UE903 UbiQuant™ Plate is a high-throughput assay for precise quantification of total ubiquitin in cell lysates using a proprietary ubiquitin binding reagent and ELISA detection.
Accurately quantitates total ubiquitin (mono- and poly-) using an anti-ubiquitin antibody.
Concentration results are obtained in µg/mL and not in arbitrary units.
The UbiQuant™ S assay is a robust tool for assessing the poly-ubiquitination levels of a protein of interest in cell lysate. This platform is based on capturing poly-ubiquitinated proteins on the plate followed by detection of the protein of interest (either native or tagged) using an antibody for the purpose of quantifying the poly-ubiquitinated forms of the protein. Detecting changes in the ubiquitination state of specific proteins in response to external stimuli has been a long and difficult process involving immunoprecipitation followed by Western blot analysis. The UbiQuant™ S kit is a quick, robust, and quantitative alternative to IP/WB analysis.
New assay plate developed for colorimetric detection allows for quantitative measurement using absorbance-based plate readers. Colorimetric readouts (650nm, 480nm) are more consistent day-to-day than luminescent-based detection methods, fast, and easy to use.
Note: This product only includes the coated plate. See UbiQuant S Kit (colorimetric) for a complete experiment (plate, antibodies, detection reagent, blocking agent).
