Cereblon complex, or CRBN complex, is an E3 Ligase that mediates ubiquitination and proteasomal degradation of target proteins. CRBN may play a role in memory and learning behaviors due to its implied interactions with KCTN1. CRBN is typically used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling. In this multi-subunit ubiquitin ligase, Cereblon acts as a substrate adaptor to brings substrate specificity without inherent enzymatic activity. Cereblon is linked to scaffolding protein Cullin 4 (Cul4a) and its regulator ring box proteins (RBX1) via DNA binding protein 1 (DDB1). The Ligase activity of the complex is determined by Cullin-RBX that catalyzes the transfer of ubiquitin from RBX bound E2 to target substrates.
This Cereblon/DDB1/Cul4A/Rbx1 complex is an active CRBN-DCX E3 ubiquitin ligase.
This complex is demonstrated to be active as E3 ligase in enzymatic ubiquitination assays, for evaluating substrate ubiquitination and PROTAC mediated ubiquitination. This complex is ideal for studying PROTAC interaction with biophysical and HTRF assays for monitoring ternary complex. In addition, this complex has Flag tagged proteins suitable for pulldown assays.
|Tag||Various, see Datasheet|
|Molecular Weight||Cereblon: 51 kDa; DDB1: 128 kDa; Cul4A: 88 kDa; Rbx1: 13 kDA|
|Quantity||10 µg, 50 µg|
|Formulation||40 mm Tris-HCL, pH 8.0, 110 mM NaCl, 2.2 mM KCl, .04% Tween-20 and 20% glycerol|
|Storage||-80°C, avoid freeze/thaw cycles|