UC107: E3LITE Ubiquitin Ligase PLATE only

LifeSensors UC107 is a 96-well E3LITE Ubiquitin Ligase Plate pre-coated with a proprietary polyubiquitin capture reagent, designed for customizable ubiquitin ligase assays in drug discovery and research.

$558.00 – $605.00

SKU UC-0107 Categories E1-E2-E3 Assays, Kits and Plates, Research Kits

Plate Size

Clear

Product Advantages

Applications

Quantitative Analysis of Ubiquitin E2 Conjugation or E3 Ligase Activity
Demonstration of novel E2 or E3 Activity
High Throughput Screening (HTS) of antagonist or agonists of ligase activity
Monitor enzyme activity during purification
Monitor E3 activity in solution phase, with the E2 of your choice
Plate-based format is amenable to high-throughput screening (HTS)
Detection system provides robust readout for E3 ligase activity
E₃LITE assay utilizes non-radioactive reporter substrates
E₃LITE assay does not require excitation in the UV range (reducing false positive rate)

The E3LITE Customizable Ubiquitin Ligase Plate consists of a plate that is pre-coated with a proprietary polyubiquitin capture reagent. The resulting product of the E3 ligase-mediated in vitro ubiquitin reaction is measured and represents a direct measure of the E3 ligase activity. The E3LITE Customizable Ubiquitin Plate represents a singular platform for the focused investigation of any E2/E3 enzyme pair, as well as many polyubiquitin linkage types. This assay has been validated for high throughput screening (HTS) and has been employed in the discovery of novel compounds that selectively modulate E3 ligase function. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Storage	All components should be stored at -80°C.
Stability	Plates must be used within 30 days.

Applications

Quantitative Analysis of Ubiquitin E2 Conjugation or E3 Ligase Activity
Demonstration of novel E2 or E3 Activity
High Throughput Screening (HTS) of antagonist or agonists of ligase activity
Monitor enzyme activity during purification
Monitor E3 activity in solution phase, with the E2 of your choice
Plate-based format is amenable to high-throughput screening (HTS)
Detection system provides robust readout for E3 ligase activity
E₃LITE assay utilizes non-radioactive reporter substrates
E₃LITE assay does not require excitation in the UV range (reducing false positive rate)