UB217H: UBE2M (Ubc12), His6

LifeSensors UB217H is a His6-tagged UBE2M (Ubc12) E2 ubiquitin-conjugating enzyme, essential for neddylation studies and ubiquitin pathway research. High purity, reliable activity.

$210.00 – $417.00

SKU UB-217H Categories E2 Ubiquitin Conjugating Enzymes, Ubiquitin Ligases and Enzymes

Product Advantages

Identify cognate E2/E3 pairs
Characterize upregulation/downregulation of E2 enzymes
Build poly-ubiquitin chains
Characterize ubiquitin chain specificity of E2 enzymes
Analyze expression of E2 enzymes

Benefits

Untagged (native) E2 conjugation enzyme

UBE2M (also known as Ubc12) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UBE2M is involved in the conjugation of the ubiquitin-like protein (Ubl) NEDD8 to target substrates (NEDDylation) along with the APPBP1/Uba3 activating enzyme. NEDDylation is required for cell cycle progression. In addition, NEDD8 is conjugated to cullin family members which are a part of SCF ubiquitin E3 ligase complexes, thereby activating them.

Info

Species	Human
Source	E. coli
Tag	His₆
Molecular Weight	21,894.2 Da
Quantity	0.8 nmol, 3.0 nmol
Concentration	40 µM
Formulation	50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles