SI4812: DUB-Resistant K48-Linked Di-Ubiquitin

DUB-resistant, K48-linked Di-ubiquitin (Ub2) contains an amino acid substitution near the C-terminus of the distal ubiquitin that renders it resistant to deubiquitylases (DUBs). DUB-resistant ubiquitin chains are ideal reagents for structural or binding studies with deubiquitylases or other ubiquitin binding proteins. They can also be used as inhibitors or to determine the linkage-specificity of DUBs. The product contains a K48R mutation in the distal ubiquitin and a wild-type C-terminus on the proximal ubiquitin.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	17,123.7 Da
Quantity	100 µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA
Storage	-80°C, avoid freeze/thaw cycles