Questions about a product, technology or experimental design? Give us a call 610-644-8845
Questions about a product, technology or experimental design? Give us a call 610-644-8845.

SI3304BP: Biotinylated K33-Linked Tetra-Ubiquitin (Phosphorylated)

LifeSensors’ SI3304BP is a biotinylated, phosphorylated K33-linked tetra-ubiquitin, perfect for studying ubiquitin signaling, protein degradation, and cellular regulation

$995.00

K33-linked ubiquitination is traditionally associated with regulation of the innate immune response. These chain types are also involved in protein stabilization and other non-degradative processes. It is a useful substrate for identifying and characterizing deubiquitinating enzymes that cleave the K33-linkage and for structural and binding studies of ubiquitin chain recognition by ubiquitin-associated domains (UBA) or ubiquitin-interacting motifs (UIMs). Phospho-ubiquitin chains represent a specialized class of polyubiquitin characterized by phosphorylation at Serine 65 and play a central role in mitophagy signaling pathways. K33 Tetra-Ubiquitin (Phosphorylated) is a tetrameric chain of wild-type ubiquitin, wherein ubiquitin monomers are enzymatically linked together via an isopeptide bond between Lysine 33 and the C-terminal Glycine. The chains are then enzymatically phosphorylated at the Ser65 position. Once the K33 Tetra Ubiquitin has been formed and phosphorylated we then biotinylate it once on one available site on the ubiquitin chain. This biotin then acts as a means of detection via streptavidin or avidin. The applications are numerous from Western Blots to ELISAs. Testing can vary based off of intended application (EX: DUB activity/specificity).   Info
Species Human
Source E. coli
Tag Biotin
Molecular Weight 34842-35082 Da (depending on degree of phosphorylation)
Quantity 25µg
Concentration Variable
Formulation 20 mM Tris pH 7.5, 150 mM NaCl
Storage -80°C, avoid freeze/thaw cycles
  • Biotinylated chains are meant to label the protein so they can be selectively separated via a multitude of methods such as Western Blotting, pull downs (see website), ELISAs, etc.
  • Can use streptavidin or avidin to visualize this protein specifically
  • Investigation of phospho-ubiquitin chain specificity and selectivity
  • Studies on the role of phospho-ubiquitin chains in protein degradation pathways (e.g., proteasomal and autophagic degradation)
  • Analysis of phospho-ubiquitin-mediated signaling pathways and cellular responses
  • Structural studies to elucidate the architecture and dynamics of phospho-ubiquitin chains
  • Screening assays to identify modulators of phospho-ubiquitin chain assembly and disassembly processes

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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PROTAC is a registered trademark of Arvinas Operations, Inc., and is used under license.

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