Specifications
K27-linked di-ubiquitin (Ub2) is a diubiquitin linked by a native isopeptide bond between the C-terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 27 on the proximal ubiquitin. The product is generated using chemical ligation. It is a useful substrate for identifying and characterizing deubiquitinating enzymes that cleave the K27-linkage and for structural and binding studies of ubiquitin chain recognition by ubiquitin-associated domains (UBA) or ubiquitin-interacting motifs (UIMs).
Info
| Species | Human |
| Source | E. coli |
| Tag | None |
| Molecular Weight | 17,112 Da |
| Quantity | 25 µg |
| Concentration | Variable |
| Formulation | 20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA |
| Storage | -80°C, avoid freeze/thaw cycles |
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