SI204: Ubiquitin-K27

SKU SI-0204-1000 Categories Ubiquitin Chains, Ubiquitin Chains and Derivatives

LifeSensors’ SI204 is a tag-free human recombinant ubiquitin K27 protein engineered with all lysines mutated to arginines except at position 27, ideal for protein conjugation studies

$184.00

In stock

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one (of seven) lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines, except at position 27. This molecule, therefore, can only form polyubiquitin chains of K27 linkage type.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	8,732 Da
Quantity	1mg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol
Storage	-80°C, avoid freeze/thaw cycles

Datasheet

Characterization of proteins responsible for ubiquitin conjugation or removal
Ligand binding studies with ubiquitin related proteins

Characterization of linkage type for target protein

Profiling of E2/E3 interactions

Characterization of E3 polyubiquitylation activity

Native (tag free) recombinant wild-type ubiquitin

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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SI204: Ubiquitin-K27

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