SI201: Ubiquitin-WT

WT-Ubiquitin is the native, unmodified ubiquitin used to study the ubiquitin-proteasome system. Ideal for assays involving E1, E2, E3 enzymes, proteasome activity, chain assembly, and DUB specificity, it serves as a control for modified variants.

$69.00

SKU SI-0201-5000 Categories Ubiquitin Chains, Ubiquitin Chains and Derivatives, Ubiquitin Derivatives

Product Advantages

Characterization of proteins responsible for ubiquitin conjugation or removal.
Ligand binding studies with ubiquitin related proteins.
Characterization of linkage type for target protein.
Profiling of E2/E3 interactions.
Characterization of E3 polyubiquitylation activity.
Native (tag free) recombinant wild type ubiquitin.

Ubiquitin is a small polypeptide that attaches via its C-terminus to lysine residues on target proteins. WT-Ubiquitin (wild-type) is the native, unmodified form and an essential tool for studying the ubiquitin-proteasome system (UPS), which controls protein degradation and turnover. It acts as the foundation for ubiquitin chains that signal for degradation or other cellular processes. WT-Ubiquitin is commonly used in assays involving E1, E2, and E3 enzymes, proteasome activity, polyubiquitin chain formation, and deubiquitinase (DUB) specificity. A single ubiquitin attachment is called monoubiquitylation, while additional ubiquitins linked through any of seven lysine residues form polyubiquitin chains. This tag-free recombinant human WT-Ubiquitin serves as a reference for modified variants and is critical for research into UPS-related diseases such as neurodegeneration, cancer, and immune disorders.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	8,564.9 Da
Quantity	5 mg
Concentration	Variable
Formulation	Phosphate Buffered Saline pH 7.4
Storage	-80°C, avoid freeze/thaw cycles