SI1104B: Biotinylated K11-Linked Tetra-Ubiquitin

K11-linked ubiquitination is traditionally associated with cell cycle regulation and proteasomal degradation. Phospho-ubiquitin chains represent a specialized class of polyubiquitin characterized by phosphorylation at Serine 65 and play a central role in mitophagy signaling pathways. K11-linked ubiquitination is traditionally associated with cell cycle regulation and proteasomal degradation. K11 linkage appears to play an important role in cell cycle signaling, as it is associated with the anaphase promoting complex (APC). K11 Tetra-Ubiquitin is a tetrameric chain of wild-type ubiquitin, wherein ubiquitin monomers are enzymatically linked together via an isopeptide bond between Lysine 11 and the C-terminal Glycine. These tetra ubiquitin are then biotinylated on an available cysteine that was mutated in one of the ubiquitins to allow for one biotin molecule to be attached. Info

Species	Human
Source	E. coli
Tag	Biotin
Molecular Weight	34759 Da
Quantity	25µg
Concentration	Variable
Formulation	20 mM Tris pH 7.5, 150 mM NaCl
Storage	-80°C, avoid freeze/thaw cycles