pE1100A: pE-SUMOstar Amp Expression Kit

Other protein expression and purification systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest. LifeSensors’ highly active and specific SUMOstar Protease 1 recognizes the tertiary structure of the SUMOstar tag, generating the desired N-terminus and never cleaving within your protein of interest. In addition, the SUMOstar system makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMOstar fusion tag and SUMOstar Protease 1, the SUMOstar system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography. This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Components:

pE-SUMOstar (T7; Amp) – 5µg SUMOstar Protease 1 (Cat. No. 4110) – 500 units SUMOstar Protease Control Protein (Cat. No. 5010) – 100µg AntiSUMO/SUMOstar Antibody (Chicken IgY, Cat. No. AB7002) – 50µg

Info

Fusion tag	His6-SUMOstar
Quantity	N/A
Promoter	T7 RNA polymerase
Selection marker	Ampicillin, Trp1
Host	E. coli, yeast
Storage	Store vector components at -20°C. Store protease and antibody components at -80°C. Avoid freeze/thaw cycles.