SI420: hSUMO1-AMC

LifeSensors SI420 (hSUMO1-AMC) is a fluorogenic substrate for SUMO1 protease assays, enabling sensitive detection of SUMO1-specific enzymatic activity in research.

$540.00

SKU SI-0420-0050 Category Sumo Derivatives Chain

In stock

Product Advantages

Applications

Used for determining the activity and specificity of SUMO-specific hydrolases, and for high-throughput screening for modulators of desumoylase activity.

SUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of human SUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing SUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (excitation at 380 nm) can be measured. The enhanced purity of SUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	11,142.5 Da
Quantity	50 µg
Concentration	Variable
Formulation	Lyophilized Powder
Storage	-80°C, avoid freeze/thaw cycles

Datasheet

Datasheet

Applications

Used for determining the activity and specificity of SUMO-specific hydrolases, and for high-throughput screening for modulators of desumoylase activity.