PY2161: pY-SUMOstar Pichia Secretory Vector

LifeSensors PY2161 is the pY-SUMOstar Pichia Secretory Vector enabling efficient expression and secretion of SUMOstar fusion proteins in Pichia pastoris yeast, boosting yield and solubility for research applications

$475.00

SKU PY-2161-0020 Categories SUMO Protease Enzymes, SUMO Protein Expression Systems, SUMOstar Protease, Yeast Tags Protein Expression, yeast

In stock

Product Advantages

Applications

Enhancing functional protein expression in P. Pastoris.

Benefits

SUMOstar fusion often enhances expression.

SUMOstar fusion often enhances protein solubility.

No known case of SUMO Protease 1 cleaving within the fused protein-of-interest.

Cleavage yields native protein with a desired N-terminus.

The pY-secSUMOstar vector enables expression and secretion of SUMOstar fusions in Pichia pastoris. Protein expression is driven by the primary alcohol oxidase (AOX1) promoter, which is both tightly regulated and highly inducible. The alpha mating factor (S. cerevisiae) targets the fusion protein to the secretory pathway, while both a FLAG and a His₆ tag preceed SUMOstar for convenient purification. Chromosomal integration is accomplished by DNA linearization within the promoter using a variety of convient endonuclease restriction sites, while multi-copy integrants can be obtained through strain selection against increasing concentrations of the antibiotic Zeocin. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

Fusion tag	His6-SUMOstar
Quantity	20 µg
Promoter	AOX1
Selection marker	Ampicillin (E. coli), Zeocin (P. pastoris)
Host	E. coli, P. pastoris
Storage	-20°C