Detection of polyubiquitylated proteins by ligand blotting
Pull down of polyubiquitylated proteins from cell lines, tissues and organs using a variety of readily available avidin supports
In situ labeling for detection of polyubiquitin by histochemistry
Replace ubiquitin antibodies for western blotting; avoid membrane boiling
Up to 1000-fold higher affinity than the single UBA domain
Avoid overexpression of affinity-tagged ubiquitins for pull downs
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state. This product is a component in Lifsensors’ UC101: E3 Lite Customizable Ubiquiin Ligase Kit.
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