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DR101 Detection Reagent 1 for UC101

DR101 is a detection reagent for UC101, enabling fast, gel-based E3 ligase assays with direct SDS-PAGE visualization—no Western blot needed.

$70.00

  • Detection of polyubiquitylated proteins by ligand blotting
  • Pull down of polyubiquitylated proteins from cell lines, tissues and organs using a variety of readily available avidin supports
  • In situ labeling for detection of polyubiquitin by histochemistry
  • Replace ubiquitin antibodies for western blotting; avoid membrane boiling
  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state.  This product is a component in Lifsensors’ UC101: E3 Lite Customizable Ubiquiin Ligase Kit. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Physical State

Liquid

Quantity

35 µL

Concentration

Variable

Storage

-80°C, avoid freeze/thaw cycles

  • Detection of polyubiquitylated proteins by ligand blotting
  • Pull down of polyubiquitylated proteins from cell lines, tissues and organs using a variety of readily available avidin supports
  • In situ labeling for detection of polyubiquitin by histochemistry
  • Replace ubiquitin antibodies for western blotting; avoid membrane boiling
  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs

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