Ubiquitin Fluorophore Assay Services
Advanced Rhodamine, AMC, and VME assay solutions for high-throughput DUB activity measurement with unparalleled precision and reliability.
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- Ubiquitin Fluorophore Assay Overview
The ubiquitin fluorophore assay utilizes a ubiquitin molecule bound to a quenched fluorophore (such as Ub-Rhodamine, Ub-AMC, or Ub-VME listed below). After the DUB digestion takes place, the fluorophore is released and fluoresces, which is detected via a plate reader. This is the simplest and most straightforward high-throughput assay to measure DUB activity.
- Service Highlights
Convenient Directional Cloning
Your gene-of-interest in frame with the SUMOpro tag
Increased Solubility
Improves protein solubility for better assay performance
Enhanced Expression
Dramatically enhances recombinant protein expression, reducing costs
Precise Tag Removal
Clean tag removal following protease cleavage
- Related Products
Ubiquitin Rhodamine
High quality fluorescent substrate for DUB assays with rhodamine detection.
- Our Comprehensive Capabilities
~35 DUBs Available
Compound Libraries
Selectivity Panels
Industry Leadership
No other company provides such a wide variety of tools for HTS and physiological assays for characterizing compounds and establishing a selectivity panel.
- Resources
DUBs Screening & Profiling Platform
Comprehensive guide to our screening capabilities and methodologies