UM504F: K63 TUBE (Fluorescein)

LifeSensors UM504F is a fluorescein-labeled K63 TUBE with 1000-10,000x specificity for K63-linked polyubiquitin, enabling precise detection in ubiquitin research.

$744.00

SKU UM-0504F-0050 Categories K63 TUBEs, TUBEs: Ubiquitin Affinity Matrix Tag Fluorescein

In stock

Product Advantages

Applications

“Far Western” blot analysis
Histo- or cyto-chemical staining. N.b. Sample fixation will require optimization.

Fluorescein-labeled (FLR) K63 TUBE binds K63-linked polyubiquitin with a 1000 to 10,000-fold preference for K63 over other linkage types and provides a sensitive and cost-effective tool for determining the abundance of K63-linked polyubiquitin in the cell, or identifying the linkage-type associated with a particular protein of interest. FLR-K63 TUBE consists of multiple ubiquitin interacting motifs separated by rigid linkers, presumably increasing its affinity to extended K63 polyUb chains. FLR- K63 TUBE is useful for detection and characterization of K63-linked polyubiquitylated proteins by ‘far Western’ blotting and in situ detection (histo- or cyto-chemical staining). FLR-K63 TUBE has one fluorescein molecule conjugated to a single, defined location within the protein.

Info

Tag	Fluorescein
Molecular Weight	10.2 kDa
Physical State	Liquid
Quantity	50 µg
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles