MG780: Molecular Glue In Vitro Ubiquitination Assay Kit

Proteolysis Targeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. Molecular Glues are naturally occurring molecules that bind an E3 and a target. This subsequently leads to ubiquitination and degradation of the target protein. The In vitro ubiquitination kit has been developed to establish a high throughput approach that can accurately predict Molecular Glue efficiency by monitoring the protein’s intrinsic ability to get ubiquitinated. We offer this kit for three E3 ubiquitin ligases: Cereblon, VHL, and HDM2 to monitor Molecular Glue mediated ubiquitination for target of choice. Molecular Glue In Vitro Ubiquitination Assay Kit Components

In Vitro ubiquitination Assay Plate	1 plate
Assay Buffer (10X)	1.2mL
E3 Mix (20X)	1 X 250µL
E1-E2-Ubiquitination Mix (20X)	1 X 250µL
Secondary Antibody (Anti-Mouse or Anti-Rabbit HRP conjugate)	60µL (100X)
Detection Reagent 1Detection Reagent 2	DR1L- 1mLDR2L- 1mL
Blocking Concentrate (5X)	5mL
Molecular Glue in DMSO (50X)	10µL
Target Protein positive control CK1α (20X)	20µL
Anti-CK1α Antibody (100X)	5µL
Anti-mouse HRP Conjugate (100X)	5µL