DR101 Detection Reagent 1 for UC101

SKU DR-0101-0035 Categories E1-E2-E3 Assays, Kits and Plates, Research Kits, TUBEs for Detection, TUBEs: Ubiquitin Affinity Matrix

DR101 is a detection reagent for UC101, enabling fast, gel-based E3 ligase assays with direct SDS-PAGE visualization—no Western blot needed.

$70.00

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state. This product is a component in Lifsensors’ UC101: E3 Lite Customizable Ubiquiin Ligase Kit. This product is protected by one or more US or Foreign patents. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Physical State	Liquid
Quantity	35 µL
Concentration	Variable
Storage	-80°C, avoid freeze/thaw cycles

Datasheet

Detection of polyubiquitylated proteins by ligand blotting
Pull down of polyubiquitylated proteins from cell lines, tissues and organs using a variety of readily available avidin supports
In situ labeling for detection of polyubiquitin by histochemistry
Replace ubiquitin antibodies for western blotting; avoid membrane boiling
Up to 1000-fold higher affinity than the single UBA domain
Avoid overexpression of affinity-tagged ubiquitins for pull downs

Domestic Delivery
All orders placed within The United States are subject to a flat shipping fee of $80. Orders are shipped via FedEx Express Standard Overnight.

International Shipping to Canada
All orders placed within Canada are subject to a flat shipping rate of $185. Orders are shipped via FedEx Express International Priority. Shipments to Canada will only be sent from our facility on Monday, Tuesday, and Wednesday to allow for adequate delivery time.

International Worldwide
All orders to be delivered to Europe, Asia, Australia and South America are subject to a flat shipping fee of $325. Orders are shipped via FedEx Express International Priority.

Customers can also use their own shipping account for deliveries if preferred. Please include the Fedex or DHL shipping account number when the order is placed or reach out to customerservice@lifesensors.com for more information.

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DR101 Detection Reagent 1 for UC101

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