IQF

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The IQF assay utilizes a pair of conjugated ubiquitin proteins. One carries a fluorophore silenced by the nearby quencher. After DUB digestion the free fluorophore is able to be detected by a plate reader. This assay gives the most physiologically accurate results because the DUB is being tested on a Ub-Ub bond.  LifeSensors has years of experience working with different DUB families, contact us for a free consultation to select the right assay for your specific project.

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