DU0121: Ub-TAMRA Reference Standard

SKU DU-0121-0025 Category IQF Diubiquitins Tags Diubiquitin, IQF

LifeSensors DU0121 UB-TAMRA Reference Standard is a high-quality 25 µg standard for ubiquitin research, ideal for proteomics and drug discovery applications. Reliable and precise

$622.00

In stock

IQF DiUb Technology LifeSensors’ diubiquitin substrates represent a new class of substrates for the continuous fluorescent measurement of true isopeptidase activity. The C-terminus of wild-type ubiquitin is conjugated via an isopeptide bond to lysine 11, 48, or 63 of a second ubiquitin molecule with the resultant diubiquitin forming an internally quenched fluorescent FRET pair (IQF). Each ubiquitin is labeled with a single molecule of either a fluorescent reporter (i.e. TAMRA) or a highly efficient quenching dye. Cleavage of the IQF DiUb by deubiquitylases leads to separation of the fluorophore from quencher and subsequent increase in observed fluorescence. The ubiquitin-TAMRA reference standard can be used to optimize and calibrate the performance of individual plate readers (or fluorometers) for measurement of the rates of hydrolysis of our IQF-DiUb by DUBs.

Info

Species	Human
Source	E. coli
Tag	None
Molecular Weight	9,161 Da
Quantity	25 µg
Concentration	40µM
Formulation	50 mM Sodium MES, pH 6.0
Storage	+4ºC

Datasheet

Determination of optimal filter, gain, and attenuation parameters for achieving maximal signal to background ratios for IQF-DiUb substrates.
Construction of calibration curves for converting RFU to molar concentrations.
Exactly matches actual product released by cleavage of LifeSensors IQF-DiUb substrates.

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DU0121: Ub-TAMRA Reference Standard

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