Mass Spectrometry Proteomics using TUBEs
Pan-selective Tandem Ubiquitin-Binding Entities (TUBEs) are engineered, high-affinity reagents composed of multiple ubiquitin-associated (UBA) domains that bind polyubiquitin chains with very high affinity. Their design enables pan-specific capture of all ubiquitin chain linkage types (K6, K11, K27, K29, K33, K48, K63, and M1)—overcoming the inherent bias of antibody-based methods like DiGly enrichment. Critically, TUBEs preserve […]