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TR-FRET in-vitro Ubiquitylation Assay

LifeSensors’ in-vitro ubiquitylation assays are awidely used high-throughput screening assay to monitor substrate ubiquitylation or E3 autoubiquitylation in a TR-FRET format.  This assay is a service only.  Contact us to learn more.

Conceptual schematic of the TR-FRET in-vitro ubiquitylation assay. Polyubiquitin chains are formed on an E3 ligase or a substrate by a standard in vitro ubiquitylation assay in the presence of FRET acceptor tagged Ub and FRET donor tagged TUBE. Polyubiquitin chains are detected by TR-FRET.

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