TR-FRET in-vitro Ubiquitylation Assay
LifeSensors’ in-vitro ubiquitylation assays are awidely used high-throughput screening assay to monitor substrate ubiquitylation or E3 autoubiquitylation in a TR-FRET format. This assay is a service only. Contact us to learn more.
Conceptual schematic of the TR-FRET in-vitro
ubiquitylation assay. Polyubiquitin chains
are formed on an E3 ligase or a substrate by a standard in vitro ubiquitylation
assay in the presence of FRET acceptor tagged Ub and FRET donor tagged TUBE.
Polyubiquitin chains are detected by TR-FRET.