Specifications
Mass spectrometry (MS) proteomics, especially when combined with TUBE technologies for protein enrichment, can be used in several innovative and impactful applications. Quantitatively profiling the ubiquitome can lead to the identification of ubiquitination sites on target proteins, and by extension unveiling the mechanisms of undiscovered E3 ligases with their substrates. MS can also inform small molecule drug discovery for these targets (e.g. PROTACs, molecular glues) by quantitatively reporting on how much target protein is degraded in the presence or absence of a drug candidate. This approach can also greatly aid the discovery and development of novel diagnostic biomarkers for challenging disease targets and development of clinically monitoring disease progression. All these applications require a simple, label-free, rapid ubiquitome approach. This kit is designed to address this for rapid yet reproducible proteomics.
*The plate is designed for research use only.
Ubiquitin Mass Spectrometry Kit Components:
| Mammalian Cell Lysis Buffer: | 5 mL Lysis Buffer, (contains protease inhibitors) (Store at -80°C) |
| UPS Inhibitor Cocktail: | 50 µL, 100X (Store at -80°C) |
| Magnetic TUBE Reagent: | 10 reactions (10 vials) (Store at 4°C) |
| Decomplexing Reagent | 50µL, 100X (Store at -80°C) |
| TUBE Wash Reagent: | 1000 µL, 10 reactions (Store at 4°C) |
| TUBE Elution Reagent: | 300 µL, 10 reactions (Store at 4°C) |
| TUBE Neutralization Reagent: | 100 µL, 10 reactions (Store at 4°C) |
| Digestion Buffer: | 5 mL, 10 reactions (Store at 4°C) |
| Reducing solution: | 1000 µL, 10 reactions (Store at -80°C) |
| Alkylating solution: | 1000 µL, 10 reactions (Store at -80°C) |
| Trypsin solution: | 20 µg, 10 reactions (Store at -80°C) |
| Gel Wash Buffer: | 20mL, 10 reactions (Store at -80°C) |
Need guidance or technical assistance with LifeSensors’ PROTAC Assay Kit and Plate? We’re happy to help! Contact our technical support team at customerservice@lifesensors.com.
Learn more about PROTACs here.
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