UM407M: K48 TUBE HF (Magnetic Beads)

$1,433.00

In stock

SKU: UM-0407M-1000 Categories: , Tags: , ,

Specifications

The use of Tandem Ubiquitin Binding Entities (TUBEs) is emerging as an indispensable strategy for ubiquitin research. The first generation of these TUBEs bind K48- and K63-linked tetraUb chains with single digit nanomolar Kds, ~100 to 1000-fold more tightly than monomeric UBAs. TUBEs also protect proteins from DUBs and the proteasome, even in the absence of inhibitors normally required to block such activity. This allows efficient isolation of native polyUb chains and attached proteins from cell lines, tissues, and organs under conditions that are less likely to alter cell physiology than those listed above. TUBE 1 and TUBE 2 have recently been demonstrated to enrich for all polyUb chain linkage types, without discrimination, making these reagents appropriate even if the linkage type is not known for the protein of interest. The K48 TUBE HF was developed to show enhanced selectivity for K48-linked polyubiquitin chains (~20 nM) over all other linkages (>2 µM). It can be used alone or in conjunction with our other TUBE products, especially K63 TUBE and M1 (linear) TUBE to investigate polyubiquitin chain linkage in your substrate protein. Magnetic-TUBEs are TUBE moieties directly coupled to magnetic beads, for the identification and characterization of polyubiquitinated proteins by western blotting and/or downstream proteomic studies. Magnetic-TUBEs facilitate convenient “one-step” pull-down of polyubiquitinated proteins.

Info

Tag

 N/A

Molecular Weight

Physical State

Liquid

Quantity

1 mL

Concentration

Variable

Storage

+4°C, avoid storing below this temperature

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Additional information

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APPLICATIONS

  • Isolation and enrichment of K48-polyubiquitinated proteins from cell and tissue extracts
  • Isolate K48-polyubiquitinated proteins for proteomic studies

BENEFITS

  • 100-fold preference for K48 poly-ubiquitin chains over all other linkages
  • TUBEs display higher affinity towards polyubiquitins than most ubiquitin antibodies
  • Avoids overexpression of epitope-tagged ubiquitin for pull downs

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