UM107: K48 TUBE HF (GST)

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Specifications

TUBEs:  A Revolution in Polyubiquitin Isolation and Characterization
The use of Tandem Ubiquitin Binding Entities (TUBEs) is emerging as an indispensable strategy for ubiquitin research. The first generation of these TUBEs bind K48- and K63-linked tetraUb chains with single digit nanomolar Kds, ~100 to 1000-fold more tightly than monomeric UBAs. TUBEs also protect proteins from DUBs and the proteasome, even in the absence of inhibitors normally required to block such activity. This allows efficient isolation of native polyUb chains and attached proteins from cell lines, tissues, and organs under conditions that are less likely to alter cell physiology than those listed above. TUBE 1 and TUBE 2 have recently been demonstrated to enrich for all polyUb chain linkage types, without discrimination, making these reagents appropriate even if the linkage type is not known for the protein of interest. K48 TUBE HF was developed to show enhanced selectivity for K48-linked polyubiquitin chains (~20 nM) over all other linkages (>2 µM). It can be used alone or in conjunction with our other TUBE products, especially K63 TUBE and M1 (linear) TUBE to investigate polyubiquitin chain linkage in your substrate protein.

Info

Tag

GST

Molecular Weight

34.6 kDa

Physical State

Liquid

Quantity

50 µg, 250 µg

Concentration

Variable

Storage

-80°C, avoid freeze/thaw cycles

This product has been cited 1 time.

Additional information

Weight

50 µg, 250 µg

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APPLICATIONS

  • Isolation and enrichment of K48-polyubiquitinated proteins from cell and tissue extracts
  • Isolate K48-polyubiquitinated proteins for proteomic studies


BENEFITS

  • Nanomolar affinity for K48 poly-ubiquitin chains
  • 100-fold preference for K48 poly-ubiquitin chains over all other linkages
  • TUBEs display higher affinity towards polyubiquitins than most ubiquitin antibodies
  • Avoids overexpression of epitope-tagged ubiquitin for pull downs

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