UM101: TUBE 1 (GST)

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Specifications

Based on UBA domains known to possess a high affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs have been demonstrated to protect polyubiquitinated proteins from both deubiquitination and proteasome-mediated degradation, allowing for the detection of relatively low abundant proteins that cannot be detected with current technologies.

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Info

Tag GST
Molecular Weight 56 kDa
Physical State Liquid
Quantity 200 µg and 1 mg
Concentration Variable
Storage -80°C, avoid freeze/thaw cycles

Additional information

Weight

200 µg, 1 mg

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Applications

  • Pull down of poly-ubiquitylated proteins from cell and tissue lysates
  • Protection of poly-ubiquitylated proteins from both deubiquitylation and degradation by the proteasome

Benefits

  • Up to 1000-fold higher affinity than the single UBA domain
  • Avoid overexpression of affinity-tagged ubiquitins for pull downs
  • Protect poly-ubiquitin chains without inhibitors specific to the proteasome or deubiquitylase activity
  • Replace ubiquitin specific antibodies for enrichment of poly-ubiquitylated proteins

 

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