Ubiquitin Enzyme Assay

UC107: E3LITE Ubiquitin Ligase PLATE only

$531.00 – $575.00

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SKU: UC-0107 Categories: ,

Specifications

The E3LITE Customizable Ubiquitin Ligase Plate consists of a plate that is pre-coated with a proprietary polyubiquitin capture reagent. The resulting product of the E3 ligase-mediated in vitro ubiquitin reaction is measured and represents a direct measure of the E3 ligase activity. The E3LITE Customizable Ubiquitin Plate represents a singular platform for the focused investigation of any E2/E3 enzyme pair, as well as many polyubiquitin linkage types. This assay has been validated for high throughput screening (HTS) and has been employed in the discovery of novel compounds that selectively modulate E3 ligase function.

This product is protected by one or more US or Foreign patents. Please read the Limited Use Label License to learn more. By purchasing this product, the purchaser agrees to comply to these terms.

Info

Storage All components should be stored at -80°C.
Stability Plates must be used within 30 days.

 

Additional information

Plate Size

96-well plate, 384-well plate
SKU

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Applications

  • Quantitative Analysis  of  Ubiquitin E2 Conjugation or E3 Ligase Activity
  • Demonstration of novel E2 or E3 Activity
  • High Throughput Screening (HTS) of antagonist or agonists of ligase activity
  • Monitor enzyme activity during purification
  • Monitor E3 activity in solution phase, with the E2 of your choice
  • Plate-based format is amenable to high-throughput screening (HTS)
  • Detection system provides robust readout for E3 ligase activity
  • E3LITE assay utilizes non-radioactive reporter substrates
  • E3LITE assay does not require excitation in the UV range (reducing false positive rate)

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