The E3LITE Customizable Ubiquitin Ligase Kit is a robust assay that allows for the quantification of the E3 ubiquitin ligase activity. The kit uses a plate that is pre-coated with a proprietary polyubiquitin capture reagent. The resulting product of the E3 ligase-mediated in vitro ubiquitin reaction is measured and represents a direct measure of the E3 ligase activity. The E3LITE Customizable Ubiquitin Ligase Kit represents a singular platform for the focused investigation of any E2/E3 enzyme pair, as well as any particular polyubiquitin linkage type**. This assay has been validated for high throughput screening (HTS) and has been employed in the discovery of novel compounds that selectively modulate E3 ligase function.
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- Ubiquitin E1 Activating Enzyme
- Choice of Ubiquitin E2 Conjugating Enzyme(s)
- E1-E2-E3 Control
- Choice of Recombinant Ubiquitin
- Detection Reagent 1
- Linear Polyubiquitin Control
- (2) modular 96- or 384-well pre-coated plates
|Storage||All components should be stored at -80°C.|
|Stability||All components, except for the pre-coated plates, are stable for at least 2 months when stored at -80°C.
Plates must be used within 30 days.
The selection of appropriate streptavidin reagent is critical to generating a robust signal. We do not recommend using avidin-horseradish peroxidase (HRP) conjugates.