UB219: UBE2T

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Specifications

UBE2T can mediate the transfer of ubiquitin from ubiquitin-activating enzyme E1 to a substrate protein or E3 ligase. This E2 enzyme has been shown to be essential for the Fanconi anemia pathway. UBE2T conjugating activity is involved with the monoubiquitylation of FANCD2, co-functioning with the E3 ligase FANCL.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 22,520.9 Da
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 25 mM Tris pH 7.4, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme