UB200 E2 Selection Panel

UB210T: UBE2D1, His6-SUMO

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Specifications

UBE2D1 is a 147 amino acid (16.6 kD) protein that mediates the degradation of abnormal proteins or proteins with short half-lives. It is a component of a Ubiquitin ligase complex including UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC, and TBL1X. It also functions in E6AP-mediated ubiquitylation of p53/TP53. UBE2D1 has been shown to interact with BARD1, UBE3A, and BRCA1.

Info

Species Human
Source E. coli
Tag His6-SUMO
Molecular Weight 28,621 Da (with tag)
Quantity 0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 50 mM Tris pH 7.5, 150 mM NaCl, 10 mM DTT, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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Applications

  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme

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