UB205: Ubc13

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Specifications

Ubc13 is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. Ubc13 acts with the ubiquitin E2 variant (UEV) protein Mms2, forming a heterodimeric complex that assembles K63-linked polyubiquitin chains utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The Ubc13/Mms2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3.

Info

Species S. cerevisiae
Source E. coli
Tag His6
Molecular Weight 17 kDa
Quantity  0.8 nmol, 3.0 nmol
Concentration 40 µM
Formulation 50mM HEPES pH 7.5, 150 mM NaCl, 10% glycerol
Storage -80°C, avoid freeze/thaw cycles

Additional information

Nanomole

0.8 nmol, 3 nmol

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  • Identify cognate E2/E3 pairs
  • Characterize upregulation/downregulation of E2 enzymes
  • Build poly-ubiquitin chains
  • Characterize ubiquitin chain specificity of E2 enzymes
  • Analyze expression of E2 enzymes

Benefits

  • Untagged (native) E2 conjugation enzyme