SU202: SUMO-2

$203.00

In stock

Specifications

Human recombinant SUMO-2 is produced in E. coli and purified to >95% homogeneity. This protein contains no extraneous tags. Vertebrates express three SUMO homologs, SUMO-1 and the closely related SUMO-2/3, which can be conjugated to substrate proteins in a manner analogous to the ubiquitin system1-3. Although a fourth SUMO (SUMO-4) has been identified in the genome, it may be an unexpressed pseudogene.

Info

Species Human
Source E. coli
Tag None
Molecular Weight 10,477.7 Da
Quantity 500 µg
Concentration Variable
Formulation  Phosphate Buffered Saline (pH 7.4)
Storage -80°C, avoid freeze/thaw cycles

Additional information

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Applications

  • For use in conjunction assays using SUMO E1 (Cat. # SU101) and UBE2I (Ubc9; Cat. # UB228H). SUMO can be conjugated to consensus sequences (ψ-K-X-D/E; ψ is any large hydrophobic residue and X is any amino acid) in vitro independent of an E3 enzyme.

Benefits

  • Inclusion of a SUMO E3 can increase the rate of conjugation and/or direct SUMOylation to nonconsensus sites.

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