SU202- 500ug SUMO-2 (human, recombinant)

$150.00

In stock

Specifications

Human recombinant SUMO-2 is produced in E. coli and purified to >95% homogeneity. This protein contains no extraneous tags. Vertebrates express three SUMO homologs, SUMO-1 and the closely related SUMO-2/3, which can be conjugated to substrate proteins in a manner analogous to the ubiquitin system1-3. Although a fourth SUMO (SUMO-4) has been identified in the genome4, it may be an unexpressed pseudogene.


Info

Accession No. P61956
Tag None
Expression Host E. coli
Purity >= 95% by RP-HPLC
Molecular weight 10,477.7 Da (calculated)
Physical State 5 mg/mL in PBS
Quantity 500 μg
Storage -80°C. Avoid repeated freeze/thaw cycles

 

Additional information

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Applications

  • For use in conjunction assays using SUMO E1 (Cat. # SU101) and UBE2I (Ubc9; Cat. # UB228H). SUMO can be conjugated to consensus sequences (ψ-K-X-D/E; ψ is any large hydrophobic residue and X is any amino acid) in vitro independent of an E3 enzyme.

Benefits

  • Inclusion of a SUMO E3 can increase the rate of conjugation and/or direct SUMOylation to nonconsensus sites.

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