Inhibits the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C) by inhibiting binding and activation by the APC activators Cdc20 and Cdh1. TAME inhibits cyclin proteolysis in Xenopus extracts (IC50 = 12 µM) and inhibits Cdc20 association with APC at 200 µM. It stabilizes cyclin B1 by prematurely terminating ubiquitination. Substrate for trypsin, thrombin, plasmin, and other proteases.
|Purity||>98% (TLC); NMR (Conforms)|
|Molecular Weight||378.9 Da|
|Physical State||White Powder|
|Solubility||May be dissolved in DMSO (35 mg/mL); or water (35 mg/mL)|
|Storage||4°C, (-20°C Long term). The product can be shipped at room temperature.|