SI520-50µg hSUMO2-AMC

$200.00

In stock

SKU: SI-0560-0050 Category: Tag:

Specifications

Human SUMO2-AMC is prepared via the conjugation of the C-terminus of mature human Small Ubiquitin-like MOdifier (SUMO)2 to 7-amino-4-methylcoumarin (AMC), leading to quenching of the intrinsic fluorescence of AMC. Upon incubation with desumoylases, AMC is released and an increase in fluorescence can be measured using at 460 nm (380 nm excitation). The high purity of LifeSensors’ SUMO2-AMC results in improved to signal:background ratios. Optimization of assay parameters and reagent requirements must be determined by the end user.

Info

Amount   50 µg
State   DMSO (liquid)
Purity   ≥ 90% by RP-HPLC
Molecular Weight   10,723 Da
Storage   -80°C in a dessicator is recommended. Avoid freeze/thaw cycles.

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Applications

  • Determining the activity and specificity of isopeptidases for Ub/Ubl proteins.
  • High Througput Screening for modulators of dSUMOylase activity.

Benefits

  • Highly sensitive compared to hSUMO2-AMC, hSUMO2-Rho110, and Z-RLRDGG-aminoluciferin
  • Allows for assaying activity at lower enzyme concentrations
  • Is active with SENP1, SENP2, and SENP6

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