SI420-50µg hSUMO1-AMC

$325.00

In stock

SKU: SI-0420-0050 Category: Tag:

Specifications

SUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of human SUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing SUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. At 380 nm) can be measured. The enhanced purity of SUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

Info

Amount 50 µg
State Lyophilized Powder
Purity >= 90% by HPLC
Molecular Weight 11,142.5 Da
Storage Dry, -20°C. Solution, -80°C. Avoid repeated freeze/thaw cycles

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Applications

  • Used for determining the activity and specificity of SUMO-specific hydrolases, and for high-throughput screening for modulators of desumoylase activity.

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