SI2902: K29-Linked Di-Ubiquitin

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SKU: SI-2902-0025 Category: Tag:

Specifications

K29-linked di-ubiquitin (Ub2) is a diubiquitin linked by a native isopeptide bond between the C-terminal glycine of the distal ubiquitin and the epsilon amino group of lysine 29 on the proximal ubiquitin. The product is generated using enzymatic ligation with high purity. It is a useful substrate for identifying and characterizing deubiquitinating enzymes that cleave the K29-linkage and for structural and binding studies of ubiquitin chain recognition by ubiquitin-associated domains (UBA) or ubiquitin-interacting motifs (UIMs).

Info

Species Human
Source E. coli
Tag None
Molecular Weight 17112 Da
Quantity 25 µg
Concentration Variable
Formulation 20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA
Storage -80°C, avoid freeze/thaw cycles

Additional information

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Applications

  • Investigation of DUB linkage specificity.

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