Increasingly, researchers are focusing on the role of poly-ubiquitin chains linked through K11. This post-translational modification has been linked to ERAD as a signal for proteasomal degradation. K11-linked chains appear to play an important role in cell cycle signaling, as they are associated with the anaphase promoting complex (APC). These diubiquitin chains are generated from the enzymatic linkage of wild-type ubiquitin through lysine 11 and purified to >95% homogenity by ion exchange chromatography.
|20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA
|-80°C, avoid freeze/thaw cycles