UM312: TUBE 2 (HRP)

$617.00

In stock

SKU: UM-0312-0050 Category: Tags: , ,

Specifications

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state. We have developed the HRP-TUBE 2 for quick and easy detection of polyubiquitin and polyubiquitinated substrates.

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Info

Tag

HRP

Molecular Weight

56.5 kDa

Physical State

Liquid

Quantity

50 µg

Concentration

Variable

Storage

-80°C, avoid freeze/thaw cycles

Additional information

SKU

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Applications

  • Detection of polyubiquitylated proteins by ligand blotting (use a 1:1000 to 1:5000 dilution for most applications)
  • In situ labeling for detection of polyubiquitin by histochemistry

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