PROteolysis TArgeting Chimeras (PROTACs) have emerged as novel therapeutics to target the undruggable proteome. PROTACs are heterobifunctional small molecules that both simultaneously bind to a target protein and a ubiquitin (Ub) E3 ligase. This subsequently leads to ubiquitination and degradation of the target protein.
LifeSensors has developed a high-throughput plate-based method to directly monitor PROTAC-mediated ubiquitination and degradation of target proteins. This method is rapid, efficient, and quantitative and works with endogenous proteins and cell lines. There is no need for stable cell line production or tagging your target protein and creating artifacts. In other words, you can create native conditions to gather the most accurate data possible.
PROTAC Assay Plate Components:
|Coated plate:||One pre-coated and blocked 96-well strip PROTAC assay plate (Store at -80°C)|
|Blocking concentrate (BC):||12 mL of 5X blocking agent for antibody dilution (Store at 4°C)|
|Plastic plate seals:||Two plate seals|
|Detection reagent 1 (DR1):||1.0 mL vial of DR1 reagent (Store at 4°C)|
|Detection reagent 2 (DR2):||1.0 mL vial of DR2 reagent (Store at 4°C)|
|MG132:||25 µL vial (10 mM in DMSO; Store at -20°C)|
|PR-619:||25 µL vial (22 mM in DMSO; Store at -20°C)|
|1,10-phenanthroline:||100 µL vial (500 mM in DMSO; Store at -20°C)|
Need guidance or technical assistance with LifeSensors’ PROTAC Assay Plate? We’re happy to help! Contact our technical support team at firstname.lastname@example.org.
Learn more about PROTACs here.